innoVation Facts – Cardiac Maturation Cues (Part 2)

In our second part of the innoVation Facts series addressing cardiac maturation cues, we focus on phenotypic changes that take place when human iPSC-cardiomyocytes (hiPSC-CMs) are cultured on FLEXcyte 96 plates to emulate the physiological conditions of the human heart.

To visualize changes in actin filament development, staining with Phalloidin was performed on Cardiosight-s (Nexel Ltd.) hiPSC-CMs plated on FLEXcyte 96 plates for 7 days and 17 days. (Scale bars = 20µM)

The staining shows a clear change in actin development from fine structures at days 7 to more pronounced filaments including typical striation, indicating the establishment of sarcomers (Fritz-Six et al., 2003; Chu et al., 2003; Ehler et al., 2004).